Molecular Biology Essay Example | Topics and Well Written Essays - 3000 words

Molecular Biology - Essay Example From the year 2011, the cases of Pertuhas have been rising in various parts of the world. Also in regions having sustained coverage of high vaccination (Pestana, 2010). In European Countries, the situation is the same, with various nations observing a rise in adults, adolescents, and infants. ECDC is taking note of the improvement and harmonization of the diagnosis for the outbreak detection, monitoring, and outbreak to assure the comparability and quality of the data. In 2011, ECDC came up with a project coordination of tasks for the surveillance of the lab of the whooping cough in EEA and Member nations. One of the reasons behind the project was to come up with a document on the guidance that related to using real time PCR on the extracted DNA from the specimens found in clinics gotten from the clients that have whooping cough. The protocol and guidance are meant for the PCR on the extracted DNA from the specimens that are suspected of contracting the whooping cough. The infection behind this is the B. parapertussis or even B. pertussis. The suitable samples are either NPS (nasopharyngeal swabs or NPA (nasopharyngeal aspirates) which were used in sampling the specimens from the patient’s posterior nasopharynx (Pestana, 2010). Other species of Bordetella like B. brondhiseptica and B.holmesii can be detected using the PCR from the extracted DNA from nasopharyngeal or nasopharyngeal swabs, and various amplification goals that were used in Bordetella are found in more than single species of Bordetella. It is crucial that the results of PCR are well interpreted. Pertussis’ clinical symptoms are typical in children who are not vaccinated. The symptoms include vomiting, coughing spams, and whooping. However, in scenarios of unvaccinated infants and neonates normally present with apnea as a symptoms. The B.pertussis infections in adults, adolescents, and older children represents contacts with the